CheKine™ Micro Urea Nitrogen Content Detection Kit - 96 T

Urea is the end product of nitrogenous compound degradation in living organisms, undergoing conversion to ammonia under the catalysis of urease. Blood urea nitrogen (BUN) is one of the principal indicators of renal function. In a sample, urea nitrogen reacts in a solution containing ferric chloride and phosphate, along with diacetyl monoxime and thiourea, upon heating to produce a red diazine compound. The intensity of the color of this compound is directly proportional to the concentration of urea nitrogen present. The determination of urea nitrogen levels is accomplished using the diacetyl monoxime method, which relies on colorimetric analysis of the reaction mixture to quantify urea nitrogen content in serum or urine specimens.