CheKine™ Micro Glucose-6-Phosphate (6PG) Content Detection K - 96 T

Glucose-6-phosphate (6PG), also known as Glucose 6-phosphate, is an intermediate product of glycolysis and pentose phosphate pathway, which is widely found in animals, plants and microorganisms. In the first step of glycolysis, glucose is catalyzed by hexokinase to form glucose-6-phosphate, which is then catalyzed by phosphate glucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis. However, in the pentose phosphate pathway, 6PG is its first substrate, and this process is also the main way to generate NADPH. In addition, 6PG can also be converted to glycogen or starch and stored. Glucose-6-phosphate dehydrogenase catalyzes the formation of glucose-6-phosphate and NADPH from 6PG and NADP+. NADPH can make WST-8 orange-yellow under the action of 1-mPMS, and the content of 6PG can be calculated by measuring the absorbance value at 450 nm.