BlockOut® Universal Blocking Buffer for Western Blotting 10- - 10 x 500 mL

BlockOut® Universal Blocking Buffer Solution for Western Blotting is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting. BlockOut® is recommended for blocking when phospho specific antibodies are used. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. BlockOut® can be used for membrane blocking and to dilute both primary and secondary antibodies. BlockOut® buffer is suitable for use with chemiluminescent and fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.