BlockOut® Universal Blocking Buffer for Western Blotting - - 500 mL

BlockOut® is an universal blocking buffer solution for Western Blotting. It is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting. Universal Blocking Buffer is recommended for blocking when phospho specific antibodies are used. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with chemiluminescent and fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.