ISG15-CHOP2 Reporter System - 30 Reactions

The ISG15-CHOP2-Reporter DeISGylation Assay consists of ISG15 fused to a reporter enzyme, as well as a separate reagent substrate for the reporter enzyme. When fused to ISG15, the reporter is rendered catalytically inactive. Following cleavage of the ISG15-reporter system by the isopeptidase, the free (and now active) reporter subsequently acts upon its substrate. Thus, in the coupled assay, the signal generated by cleavage of the reporter enzyme’s substrate is a quantitative measure of isopeptidase activity.-\n

Contents

-\n-- ISG15-CHOP2-Reporter System: 1 x 375 μL (4 μM)-\n-- PLpro (Control Isopeptidase): 2 x 10 μL (2 μM)-\n-- Reporter Substrate 2: 1 x 30 μL (25 μM)-\n-- Control Fluorophore 2: 1 x 20 μL (1 mM)