Di-Ubiquitin K63 (IQF, Position-5) - 50 µg

IQF DiUb Technology-\nLifeSensors’ diubiquitin substrates represent a new class of substrates for the continuous fluorescent measurement of true isopeptidase activity. The C-terminus of wild type ubiquitin is conjugated via an isopeptide bond to lysine 48 (K48) of a second ubiquitin molecule with the resultant diubiquitin forming an internally quenched fluorescent FRET pair (IQF). Each ubiquitin is labeled with a single molecule of either a fluorescent reporter (i.e. TAMRA) or a highly efficient quenching dye. Cleavage of the IQF DiUb by deubiquitylases leads to separation of the fluorophore from quencher and subsequent increase in observed fluorescence.-\n-\nIQF DiUb Variants-\nThere is a remarkable difference between K48- and K63-linked ubiquitin molecules in their three-dimensional structure, which accounts for their individual functions. Beyond differential linkages (e.g. K48, K63, K11), LifeSensors has created subpanels of IQF diubiquitin substrat