qPCR

When Your qPCR Must Detect What Others Miss  

 

Takyon® qPCR kits from Eurogentec are formulated for fast, accurate, and consistent amplification. Each combines a high-affinity DNA polymerase with an optimized buffer to ensure robust amplification across a wide dynamic range:

  • For DNA and RNA detection
  • SYBR® and probe technologies
  • Compatible options for every thermocycler
  • Multiplexing up to 5 targets (Takyon® Ultra)

 

Takyon Master Mixes

1. Takyon®: delivers dependable qPCR amplification with versatile formats tailored to routine applications.

  • Available in SYBR® and probe-based formats
  • Blue visualization option to reduce pipetting errors
  • Multiple ROX™ configurations: High / Low / None
  • dUTP/UNG option for carryover contamination control
  • All dTTP-based mixes can be converted into One-Step RT-qPCR kits using the One-Step Kit Converter 

2. Takyon® Ultra: provides enhanced sensitivity and high inhibitor tolerance, ideal for complex matrices (e.g., blood, tissue extracts, environmental samples) and high-performance assays.

Available as:

  • Superior inhibitor tolerance for complex biological matrices
  • Expanded multiplexing capacity (up to 5-plex)
  • Separated ROX™ vial for universal thermocycler compatibility
  • Enhanced sensitivity and performance, powered by an optimized 4X formulation
  • Stable for at least 24 hours at 20 °C

Select The Optimal Formulation for Your Workflow:

Choosing the right Takyon® mix depends on:

1. Detection technology: SYBR® vs probe-based
2. The optimal Master Mix reference
3. ROX™ requirement of your thermocycler
4. Additional workflow options: Blue dye, dUTP/UNG, One-Step conversion

1. Choose Your Detection Technology

2. Identify the Master Mix References According to ROX™ Level and Additional Options

Takyon® Products
Assay type ROX™ LOW ROX™ NO ROX™ Concentration Additional options
Takyon® SYBR® MMx UF-RSMT-B0701 UF-LSMT-B0701 UF-NSMT-B0701 2X Blue dTTP
Takyon® SYBR® Core kit UF-NSCT-B0701 2X Blue dTTP
Takyon® probe MMx UF-RPMT-C0701 UF-LPMT-C0701 UF-NPMT-C0701 2X dTTP
Takyon® probe MMx (UNG) UF-RPMU-C0701 UF-LPMU-C0701 UF-NPMU-C0701 2X dUTP‑UNG
Takyon® probe MMx Blue dTTP UF-RPMT-B0701 UF-LPMT-B0701 UF-NPMT-B0701 2X Blue dTTP
Takyon® probe Core kit UF-RP5X-C0501 UF-LP5X-C0501 UF-NP5X-C0501 5X dTTP
Takyon® probe Core kit UF-RPCT-C0201 UF-NPCT-C0201 2X dTTP

Every dTTP kit can be converted to a One-Step RT-qPCR kit adding the One-Step kit converter. 

3. Determine the Level of ROX™ Needed According to Thermocycler

Takyon® Products
No ROX™ Low ROX™ ROX™
Agilent: AriaMx, AriaDx
Analytik Jena: qTOWER³ systems, qTOWERiris systems
Bio-Rad: CFX systems
Qiagen: QIAquant® systems, Rotor-Gene® systems
Roche: LightCycler® 96 & 480 & PRO
bms: Mic qPCR Cycler
Most other instruments 		Applied Biosystems: 7500 systems, QuantStudio™ systems, ViiA™7 systems Applied Biosystems: 7900HT systems, StepOne™, StepOnePlus™

Every dTTP kit can be converted to a One-Step RT-qPCR kit adding the One-Step kit converter. 

4. Additional Options

Takyon® Products
Assay type ROX™ LOW ROX™ NO ROX™ Concentration Additional options
Takyon® SYBR® MMx UF-RSMT-B0701 UF-LSMT-B0701 UF-NSMT-B0701 2X Blue dTTP
Takyon® SYBR® Core kit UF-NSCT-B0701 2X Blue dTTP
Takyon® probe MMx UF-RPMT-C0701 UF-LPMT-C0701 UF-NPMT-C0701 2X dTTP
Takyon® probe MMx (UNG) UF-RPMU-C0701 UF-LPMU-C0701 UF-NPMU-C0701 2X dUTP‑UNG
Takyon® probe MMx Blue dTTP UF-RPMT-B0701 UF-LPMT-B0701 UF-NPMT-B0701 2X Blue dTTP
Takyon® probe Core kit UF-RP5X-C0501 UF-LP5X-C0501 UF-NP5X-C0501 5X dTTP
Takyon® probe Core kit UF-RPCT-C0201 UF-NPCT-C0201 2X dTTP
  • Takyon® Blue Additive is an inert blue dye to improve Master Mix visualization and avoid dispensing errors. Most Takyon® kits already have blue dye included.
  • dUTP/UNG Additive can be added in any dTTP mix to make the kit compatible with carryover contamination prevention.
  • One-Step Kit Converter: transforms any Takyon® dTTP mix into a One-Step RT-qPCR kit.

From Routine to Complex qPCR, Takyon® Delivers Every Time

Together, Takyon® and Takyon® Ultra provide a complete, instrument-compatible and performance-driven qPCR solution, from routine assays to the most demanding, inhibitor-rich and multiplexed workflows.

Our technical team can guide you to the optimal Takyon® formulation for your thermocycler and application.

Enhance Your Workflow with Recommended Accessory Products

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Why Choose Tebubio & iXCells

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  • Reference

    Figure 1: Courtesy of iXCells, your trusted supplier for iPSCs.

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