EZ-QC™ mRNA Assay Kits
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The only assay kits on the market for mRNA quality control. Efficient translation of mRNA to protein critically depends on correct modifications such as 5’-capping and 3’-poly(A) tailing. These are essential quality attributes that influence stability, expression, and overall performance of mRNA. Traditional biophysical techniques (e.g., LC-MS) are often complex, costly, and time-consuming. As the demand for mRNA products increases, so does the need for easy-to-use, reliable QC methods. Tebubio now offers EZ-QC™ mRNA Cap 1 Efficiency, Capping Efficiency, and Poly(A) Tail Length Assay Kits, simple, cost-effective tools that integrate seamlessly into standard laboratory workflows. |
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EZ-QC™ mRNA Cap 1 & Capping Efficiency Assay Kits
Key benefits:
- Low input: 12 pmol for Cap 1 Efficiency Kit, 5 pmol for Capping Efficiency Kit
- Fast workflow: Complete analysis in a single day
- Cost-effective: Uses standard PAGE lab equipment (no LC-MS required)
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Figure 1: Cap 0, Cap 1, and mixed samples assessed with the EZ-QC™ mRNA Cap 1 Efficiency Assay Kit. Gel imaging software quantifies the % of Cap 0 and Cap 1 mRNA. |
Figure 2: Capped and uncapped 5’-end fragments resolved by PAGE for capping efficiency analysis with standard equipment. |
EZ-QC™ mRNA Poly(A) Tail Length Assay Kit
Polyadenylation at the 3’-end is a critical modification influencing mRNA stability and protein expression. The EZ-QC™ Poly(A) Tail Length Assay Kit resolves a wide variety of tail lengths in a single run, ensuring your constructs are optimized for maximum expression.
Figure 3: RNase A-resistant poly(A) tail of treated mRNA appears as a defined smear on PAGE. Lane 1: Poly(A) 20-mer ladder; Lane 2: poly(A) tail of treated mRNA.
Why choose EZ-QC™?
Tired of long wait times and high costs of outsourcing QC with LC-MS?
With EZ-QC™ Assay Kits, you can perform quantitative 5’-capping efficiency and 3’-poly(A) tail length analysis in-house, using only standard PAGE equipment:
- Fast benchtop assays for both 5’- and 3’-end QC
- Low-input mRNA requirements (picomoles, not micromoles)
- Simple PAGE-based workflow
- Highly cost-effective – more experiments for your budget
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References
Images: Coutesy of CellScript our trusted partner.
Want faster, easier mRNA QC at your bench?
Let’s discuss how EZ-QC™ Assay Kits can fit into your project and save you time and resources.