Scientific Library

For more than 2 years now, the Silicon Rhodamine-like (SiR) technology has allowed the live cell imaging field with fluorescence microscopy to evolve significantly.

Fluorescent

Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is

As you may already know, Spirochrome have developed a broad range of Live Cell Imaging probes for the staining of DNA, Lysosome, F-Actin

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient

Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target

Following on from the popular SiR-Probes, earlier this year Spirochrome introduced new SPY probes, as presented in

Despite its importance in the regulation of many cellular processes, membrane tension is still complicated to measure. And despite valuable insights, the techniques currently available remain complex and

With the great success of the SIR-probes for efficient labeling of the cell Cytoskeleton, DNA and Lysosome, Spirochrome have just