CheKine™ Mirco Neutral Invertase (NI) Activity Assay Kit - 96 T

Invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose, and is one of the key enzymes in sucrose metabolism in higher plants. According to the optimal pH, higher plant Ivr can be divided into acidic invertase (AI) and Neutralinvertase (NI) types. CheKine™ Micro Neutral Invertase (NI) Activity Assay Kit can detect plant tissues samples. In this kit, NI catalyzes sucrose decomposition to produce reducing sugar, which further reacts with 3,5-dinitrosalicylic acid to produce brown-red amino compounds, which have characteristic light absorption at 540 nm, and the light absorption value at 540 nm is proportional to the amount of reducing sugar generation in a certain range. NI activity was calculated by increasing rate of light absorption.