CheKine™ Mirco NAD Malic Enzyme (NAD-ME) Activity Assay Kit - 96 T

Malic enzyme (ME) is widely present in the cytoplasm of microorganisms, cultured cells, animals, and plants, especially with high activity in plant tissues. ME catalyzes the reversible oxidation decarboxylation of malic acid, producing pyruvate and CO2, as well as the reduction reaction accompanied by NAD(P)+, which is a key enzyme in malic acid metabolism. ME activity is closely related to biosynthesis and antioxidant activity. Based on the specificity of coenzyme and substrate, ME can be divided into NAD-ME (EC1.1.1.38) and NADP-ME (EC1.1.1.40). NAD-ME can catalyze the reduction of NAD+ to NADH, and the rate of NADH increase at 340 nm can reflect its activity.