CheKine™ Mirco Granule-Bound Starch Synthase (GBSS) Activity - 96 T

Granule-bound starch synthase (GBSS, EC2.4.1.21) exists in the starch body in a bound state, catalyze the starch chain elongation reaction, and is mainly responsible for the synthesis of amylopectin. CheKine™ Micro Granule-Bound Starch Synthase (GBSS) Activity Assay Kit can detect plant tissues. In this kit, GBSS catalyzes the reaction between ADPG and starch primers (glucan), transfers glucose molecules to starch primers, and generates ADP at the same time. Furthermore, the addition of pyruvate kinase, hexokinase and glucose 6-phosphate dehydrogenase in the reaction system catalyzed the reduction of NADP+to NADPH in turn, where the amount of NADPH generated was proportional to the amount of ADP generated in the previous reaction. GBSS activity could be calculated by measuring the increase of NADPH at 340 nm.