CheKine™ Mirco Acid Phosphatase (ACP) Activity Assay Kit - 96 T

ACP (Acid Phosphatase) catalyzes the hydrolysis of phosphomonoesters into inorganic phosphate under acidic conditions, commonly found within lysosomes of macrophages. ACP is frequently employed as an auxiliary diagnostic tool for prostate cancer. CheKine™ Micro Acid Phosphatase (ACP) Activity Assay Kit provides a simple, convenient, and rapid method for determining ACP activity, applicable to a variety of samples including plant and animal tissues, serum, and plasma. Its principle relies on the ACP-catalyzed hydrolysis of sodium phenyl phosphate to produce phenol in an acidic milieu; subsequently, phenol reacts with 4-aminoantipyrene and potassium ferricyanide to form a red quinone derivative which exhibits characteristic light absorption at 510 nm. By measuring the rate of increase in absorbance at 510 nm, the ACP activity can be calculated.