Ac-Asp-Met-Gln-Asp-AFC (Caspase 3 Substrate) - 5 mg

Molecular Weight: 760Salt Form: N/APurity: >95%Sequence (3-letter): Ac-Asp-Met-Gln-Asp-AFCSequence (1-letter): Ac-DMQD-AFCStorage: -20 °C or belowAc-Asp-Met-Gln-Asp-AFC is a fluorogenic substrate for Caspase 3 (Apopain). Caspase 3 is a cysteine protease that is rapidly activated during apoptosis and that cleaves several substrates including poly(ADP-ribose) polymerase (PARP). Caspase 3 is activated by Caspases 8 9 and 10 and cleaves and activates caspase 6 and 7. Caspase 3 cleaves the amyloid precursor protein (APP) contributing to Amyloid beta formation and has been implicated in Alzheimer’s disease and proposed as a target for drug discovery.Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.References1. Talanian R.V. et al. (1997) “Substrate Specificities of Caspase Family Proteases” J. Biol. Chem. 272 (15): 9677–9682.2.Nagane M. et al. (1998) “Drug resistance of human gl