Ac-Asp-Glu-Val-Asp-pNA (Caspase-3 (Apopain) Substrate) - 25 mg

CAS Number: 189950-66-1Molecular Weight: 638.22Salt Form: TFAPurity: >96%Sequence (3-letter): Ac-Asp-Glu-Val-Asp-pNASequence (1-letter): Ac-DEVD-pNAStorage: -20 °C or belowSolubility: DMSO and acetonitrile:methanol (1:1) to 1 mg/mlCaspase-3 (Apopain) Substrate is a colorimetric substrate for caspase-3 with the sequence Ac-DEVD-pNA. Caspase-3 is a protease that is rapidly activated during apoptosis and that cleaves several substrates including poly(ADP-ribose) polymerase (PARP). Cleavage of Ac-DEVD-pNA is monitored by measuring absorbance at 405 nm. Ac-DEVD-pNA is poorly cleaved by caspase-2.Km = 18 μM (Casp-1) 11 μM (Casp-3) 32 μM (Casp-4) 180 μM (Casp-6) 12 μM (Casp-7)References1. Talanian R.V. et al. (1997) “Substrate Specificities of Caspase Family Proteases” J. Biol. Chem. 272 (15): 9677–9682.2.Nagane M. et al. (1998) “Drug resistance of human glioblastoma cells conferred by a tumor-specific mutant epidermal growth factor receptor through modulation of Bcl-X