Ac-Asp-Glu-Val-Asp-AMC (Caspase 3 (Apopain) Substrate) - 25 mg

CAS Number: 169332-61-0Molecular Weight: 675.24Salt Form: TFAPurity: >95%Sequence (3-letter): Ac-Asp-Glu-Val-Asp-AMCSequence (1-letter): Ac-DEVD-AMCStorage: -20 °C or belowAc-Asp-Glu-Val-Asp-AMC is a fluorogenic substrate for Caspase 3 (Apopain). Caspase 3 is a cysteine protease that is rapidly activated during apoptosis and that cleaves several substrates including poly(ADP-ribose) polymerase (PARP). Caspase 3 is activated by Caspases 8 9 and 10 and cleaves and activates caspase 6 and 7. Caspase 3 cleaves the amyloid precursor protein (APP) contributing to Amyloid beta formation and has been implicated in Alzheimer’s disease and proposed as a target for drug discovery.Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.References1. Talanian R.V. et al. (1997) “Substrate Specificities of Caspase Family Proteases” J. Biol. Chem. 272 (15): 9677–9682.2.Nagane M. et al. (1998) “Drug resis