Ac-Ala-Glu-Val-Asp-pNA (Caspase 3 Substrate) - 5 mg

Molecular Weight: 594.23 Salt Form: N/A Purity: >96% Sequence (3-letter): Ac-Ala-Glu-Val-Asp-pNA Sequence (1-letter): Ac-AEVD-pNA Storage: -20 °C or belowAc-Ala-Glu-Val-Asp-pNA is a colorimetric substrate for Caspase 3. Caspase 3 is a cysteine protease that is rapidly activated during apoptosis and that cleaves several substrates including poly(ADP-ribose) polymerase (PARP). Caspase 3 is activated by Caspases 8 9 and 10 and cleaves and activates caspase 6 and 7. Caspase 3 cleaves the amyloid precursor protein (APP) contributing to Amyloid beta formation and has been implicated in Alzheimer’s disease and proposed as a target for drug discovery.Activity is quantified by the release of p-nitroaniline which is measured by absorbance at 405 nm.References 1. Talanian R.V. et al. (1997) “Substrate Specificities of Caspase Family Proteases” J. Biol. Chem. 272 (15): 9677–9682.2.Nagane M. et al. (1998) “Drug resistance of human glioblastoma cells conferred by a tumor-specific muta