6-Carboxy-H2DCFDA (6-carboxy-2',7'-dichlorodihydrofluorescei - 25 MG

6-Carboxy-H2DCFDA is a chemically reduced, acetylated form of fluorescein used as an indicator for reactive oxygen species (ROS) in living cells. Initially non-fluorescent, it becomes green-fluorescent after intracellular esterases remove the acetate groups and ROS oxidize it within the cell. The resulting 6-carboxy-2’,7’-dichlorofluorescein emits bright fluorescence in the green channel (with an absorption maximum at 511 nm and an emission maximum at 533 nm).