SDT1 (Pyrimidine 5'-nucleotidase) - 100 ug

Recombinant SDT1 (Pyrimidine 5’– nucleotidase) C-terminal His-tagged fusion protein from S. cerevisiae. Purified from E.coli using Ni-NTA column chromatography. Pyrimidine 5’- nucleotidase catalyzes the hydrolysis of the pyrimidine monophosphates (CMP and UMP) into their respective nucleoside and free phosphate (PO4).Product Background:SDT1 pyrimidine 5’– nucleotidase catalyzes the hydrolysis of the pyrimidine monophosphates (CMP and UMP) into their respective nucleoside and inorganic free phosphate (PO4).[1] The enzyme is dependent on magnesium ions (Mg 2+) for activity and is susceptible to common chelating agents such as EDTA. In addition the enzyme demonstrates a maximum activity between pH 7.5 to 8.0.Multiple forms of pyrimidine 5’- nucleotidases have been identified in mammals and vary by substrate specificity distribution cellular localization and regulation. Dephosphorylation of nucleotides to nucleosides serves a regulatory control. For example hereditary d