Human BCRP vesicle kit (HEK293 derived) - 100 reactions / kit (based on 96-wells format)

Human drug efflux transporters (ABC transporters) are transiently overexpressed in human embryonic kidney (HEK293) cells. Inside-out membrane vesicles are prepared from these HEK293 cells and used for high-throughput applications. The transporters translocate substrates into membrane vesicles in an ATP-dependent manner. Detection of the substrate inside the vesicles is possible with radiolabel, fluorescent or LC-MS detection. Cell4Pharma delivers high quality large uniform batches with guaranteed transport ratios. Each vesicle kit contains enough of the following materials for a 100 reactions: •Vesicle vial (100 reactions) •Cell4Pharma AMP-ATP (100 mM) •Cell4Pharma assay buffer •Cell4Pharma Stop/Wash solution