hMSC adipogenesis ind. medium, basal - 500 ml

The hMSC adipogenesis induction medium is a sterile liquid culture medium for inducing adipogenic differentiation of human mesenchymal stem cells (hMSC). The medium is delivered as a basal medium and is suitable for culturing hMSC after adding the supplement kit components. The formulation is optimized for initial seeding of 6,000 cells / cm² up to confluence (approx. 90 %). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. After adding 50 ml FCS, 10 ml HEPES and 5 ml L-Glutamine and optional 3,5 ml antibiotics to the 500 ml hMSC adipogenesis induction medium basal the media can be stored in the dark at 2°C to 8°C for up to 4 weeks. After adding the induction factor Dexamethasone, Indomethacine, 3-Isobutyl-1-methyl-xanthine and insulin to the media, the media can be stored in the dark at °C to 8°C for up to 1 week. Therefore each a