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To dilute or not to dilute biological samples? And if yes, how much? That's the question!

Following our post on whether samples should be pooled or not when performing studies for

Unfortunately, and still in the XXIst century, micronutrient (MN) malnutrition is a problem in all countries, in all segments of society. While part of the world starves, another part is obese. And a balanced

Back to dilution of samples. After our post on whether samples should be diluted or not, let's discuss today about how much they should be diluted.

Case # 1 - ELISA or simplex

Periodontitis, also generally called gum disease or periodontal disease, begins with bacterial growth in the mouth and may end, if not properly treated, with tooth loss due to destruction of the tissue

ELISAs are widely used in biomarker-related studies, especially when a high number of samples is involved, and only a few biomarkers need to be quantified. That said, incubations can be somehow cumbersome,

Hyaluronic Acid (HA) is important in many biological processes such as wound repair, tissue hydration and inflammation. HA is also a potential biomarker for diseases such as osteoarthritis, liver cirrhosis

Depending on the phase where your biomarker-related research program is,

Gene expression is regulated by different mechanisms. One of them is the binding of

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocorticotropic hormone. Corticosterone is a major indicator of stress in non-human mammals.

Biomarkers specialists are often asked to select an ELISA kit for researchers: with thousands of ELISA references available on the market, the choice can be tricky regarding proteins for which several

The neuropeptides, Oxytocin and Vasopressin, were isolated and synthesized by Vincent du Vigneaud at Cornell Medical College in 1953, work for which he received the Nobel Prize in Chemistry in 1955. Oxytocin

Chronic Mucosal Inflammation is the hallmark of common airway diseases (ex.  Allergic Rhinitis and asthma). Lipoxin B₄(LXB₄) is an endogenous mucosal protective mediator decreasing