Autotaxin Inhibitor Screening Kit - EA KIT

The Autotaxin Inhibitor Screening Kit utilizes purified autotaxin (ATX) and the autotaxin substrate FS-3 which fluoresces upon cleavage. The assay is performed in a 96 well plate and quantifies the autotaxin activity in the presence of the user's samples by measuring changes in fluorescence over time.Sample Type: Inhibitors against ATXSample Volume: 10 µL/ well of your sample compoundsSample Considerations: Some organic solvents interfere with the assay (see technical data sheet).Product BackgroundThe Autotaxin Inhibitor Screening Kit developed by Echelon Biosciences Inc. uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence however once autotaxin cleaves FS-3 the fluorophore becomes liberated from the quencher resulting in increased fluorescence.Percent autotaxin inhibition is determined by referencing no inhibitor control