TNKS2 Homogenous Assay Kit - 384 rxns.

The TNKS2 (also known as PARP5b) Homogeneous Assay Kit is designed to measure TNKS2 activity for screening and profiling applications. TNKS2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to its substrate. The key to the TNKS2 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for TNKS2 reactions. First, a sample containing TNKS2 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.