Suppl. Kit for EC prolif. medium, FCS - 500 ml

The supplement kit for endothelial cell proliferation medium is suitable for culturing Provitro human endothelial cells after adding the supplement kit components to 500 ml basal medium The formulation is optimized for initial seeding of 4,000 cells / cm² up to confluence (approx. 90 %). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. Store the supplements at -20°C. Do not use the supplements after expiring date. The supplement kit for endothelial cell priliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time.