Green 3CLglow 3CLpro assay - 60mL - 60mL

Measure 3CL protease (3CLpro) inhibition or screen for inhibitors with the live cell, fluorescent 3CLglow assay. The 3CLglow sensor expresses a green fluorescent protein in the presences of the 3CLpro enzyme as well as a constitutively expressed red fluorescent protein to monitor cell health and compound toxicity. The assay is packaged in BacMam for expression in mammalian cells. BacMam provides even, near 100% transduction efficiency in HEK293 cells as well as expression in many other cell lines, iPSCs, and primary cultures including A549s and HUVECs. Expression is easily titrateable to your experimental needs or to adjust assay sensitivity. This kit includes the 3CLglow sensor BacMam, 3CLpro enyme BacMam, and 500mM sodium butyrate in H2O. 5mL and 30mL sizes are also available.