THUNDER TR-FRET Assay Kits: High-Performance Assays Without the Premium Price

Why Pay More for TR-FRET Assays?

 

With Thunder™ TR-FRET Kits, you get:

  • Superior sensitivity & reliability as leading TR-FRET brands
  • Proven validation in cellular signaling assays
  • Flexible kit sizes (100 to 20.000 points) to fit your workflow
  • Significant cost savings → sustainable for routine use

Enhanced TR-FRET Made Simple

Thunder™ uses an optimized donor–acceptor pair (Europium + Far-Red fluorophore) to deliver:

  • Wash-free, homogeneous workflow → faster results
  • Low background & high sensitivity → reliable data
  • Long signal stability → multiple readings & archiving

✅ Optimized antibody concentrations ensure superior S/B ratios vs. standard TR-FRET assays.

 

Cryopreserved Format = Total Flexibility

Forget the hassle of long-term cell maintenance. With Transiport cells:

  • Cells are readily available when you need them
  • Assay prep is fast and straightforward - just thaw, seed, and go
  • Use normal condition culture (5 % CO2, humidity), no need to block an incubator at 8% or low humidity
  • Complete your experiment in as little as two days

This streamlined workflow lets you plan experiments on your schedule, without compromising on data quality.

Built-In Accuracy: Ratiometric Measurement for Reliable Results

Another major advantage of TR-FRET is the ability to analyze data not only from the 665 nm emission signal, but also using the 665/615 nm ratio. This ratiometric measurement improves reproducibility by correcting small pipetting errors and minimizing compound-related artifacts.

Some test compounds may reduce the donor signal at 615 nm (for example, through light absorption, scattering, or the inner filter effect). By using the 665/615 nm ratio, Thunder™ TR-FRET automatically compensates for these issues, ensuring cleaner, more reliable assay results.

 


Figure 1: The binding of Eu-labeled and FR-labeled antibodies to the target protein in the cell lysate brings donor and acceptor molecules into close proximity.

Upon excitation of the Europium chelate at 320 or 340 nm, energy is transferred to the far-red acceptor fluorophore, which in turn emits light at 665 nm.

The intensity of light emission at 665 nm is proportional to the level of protein.

 

Proven in Cellular Signaling Assays

Thunder™ is validated in real-world cell models, showing robust performance and competitive advantage:

 

Phospho-IRβ (Y1150/Y1151) in B lymphocytes
→ The only TR-FRET cellular assay with pharmacological validation data.

 

Phospho-ERK1/2 (T202/Y204) in HEK293 cells
→ Outperformed a competitor TR-FRET kit with a higher S/B ratio.

 

Figure 2:  Modulation of phospho-Insulin Receptor β (Y1150/Y1151) in B lymphocyte cells.
The only cellular phospho-IRβ assay showing pharmacological validation data.

Figure 3: Stimulation of phospho-ERK1/2 (T202/Y204) in HEK293 cells
BioAuxilium's assay outperforms a competitor's TR-FRET assay in terms of S/B ratio.

 

These results give scientists the confidence to adopt Thunder™ for routine screening. 

Why Choose Thunder™ with Tebubio: Trusted Science. Smarter Pricing.

Compared to competitors kits, Thunder™ offers:

  • Same assay performance, 30–40% more cost-effective
  • Flexible pack sizes (100 to 20.000 points) → adapt to your projects
  • Novel assays covering targets not always addressed by competitors
  • Supported by Tebubio’s scientific experts in Europe

Adopt Thunder™ as your TR-FRET solution and stretch your R&D budget further.

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  • References

    Image: Coutesy of BioAuxilium, your trusted partner for Förster resonance energy transfer technology.

See the Results for Yourself

Ready to try Thunder™ TR-FRET? Join leading scientists already using Thunder™ for routine, reproducible assays.

Order your kit today and experience the same reliability, with sustainable pricing.