Expertise and flexibility: Customize your mRNA to your exact needs
Our experience allows us to tailor mRNA production to the specific requirements of each project, with a strong focus on achieving efficient transcription, translation, and product quality. From DNA template selection and sequence optimization to purification, capping, nucleotide modification and quality control, our flexible workflow can be adapted to the needs of your research and development program.
We provide research grade (RUO) RNA from 100 µg to 10 mg, for mRNA lengths of up to 10 kb, with multiple options available to customize your product.
Sequence Optimization
Quality Controls
From DNA template to optimized mRNA
Our mRNA synthesis process is compatible with a broad range of DNA starting materials, including customer-provided circular plasmids and linear DNA. Alternatively, you can use our optimized plasmid incorporating enhanced UTRs and a tailored poly(A) tail to support efficient mRNA production and protein expression in mammalian cells.
DNA Template & Sequence Optimization
Whether using our DNA templates or yours, we produce and deliver RNA matching your specific requirements. Your template sequences are stored, to let you order new batches quickly and cost-effectively.
Sequence optimization is an integral part of our approach. We perform codon optimization to enhance protein expression while reducing the potential for immunogenic sequences and minimizing the formation of truncated mRNA products.
Fig 1. Size and integrity by capillary electrophoresis of an mRNA without (left panel) or with codon optimization (right panel)
Tailored capping and nucleotide modifications
Our flexible mRNA workflow enables the incorporation of a wide range of modifications to meet the requirements of your application. Depending on your needs, mRNA can be produced with Cap1, Cap0 or uncapped, using either co-transcriptional or enzymatic capping.
Modified nucleotides, including N1-methylpseudouridine (N1-Me-Ψ), 5-methoxyuridine (5moU), pseudouridine (Ψ) and 5-methylcytidine (5meC), can be incorporated to reduce mRNA immunogenicity.
mRNA Production
nucleotides or enzymes
For applications requiring mRNA tracking, the integration of nucleotides labelled with fluorescent dyes is also possible, enabling visualization of mRNA delivery and intracellular localization.
Combining a reporter mRNA (e.g. eGFP) with a fluorescently labelled nucleotide (e.g. Cy5) provides information on both mRNA delivery and protein expression within the same cellular system.
Fig 2. HEK293 cells transfected with eGFP mRNA labeled with Cy5 fluorophore (above) compared to unlabeled control (below).
Purification and quality control
RNA purification is performed using silica-coated magnetic beads in a fully automated process. Additional purification steps can be performed upon request, including oligo(dT)-based selection of poly(A)-tailed RNA and dsRNA removal.
RNA quality is assessed at multiple levels, including purity, size and integrity, with additional QC analyses such as endotoxin level, dsRNA content and poly(A) tail length available upon request.
Purification & Quality Controls
Ready to advance your RNA project?
Whether you're developing vaccines, oncology therapeutics or protein replacement strategies, Tebubio supports every stage of your workflow, from custom RNA production for research and preclinical applications to delivery and biological validation.
End-to-end solutions: from RNA to biological validation
Tebubio supports every stage of your RNA project with integrated solutions designed to streamline the transition from RNA production to in vivo applications.
Why Choose Tebubio as Your Laboratory Service Provider
EXPERTISE
PhD-level expertise
Scientific guidance tailored to your research question.
FLEXIBILITY
Support at the right scale
From a single assay to a complete study, with solutions that evolve with your project.
SIMPLICITY
One partner, less complexity
From study design to results, with a dedicated project manager by your side.
ACCESSIBILITY
European expertise, global reach
Your research, handled in France, with close collaboration and support for researchers in Europe.
Why Choose Tebubio for Custom RNA Production?
Why Choose Tebubio as Your Laboratory Service Provider
EXPERTISE
PhD-level expertise
Scientific guidance tailored to your research question.
FLEXIBILITY
Support at the right scale
From a single assay to a complete study, with solutions that evolve with your project.
SIMPLICITY
One partner, less complexity
From study design to results, with a dedicated project manager by your side.
ACCESSIBILITY
European expertise, global reach
Your research, handled in France, with close collaboration and support for researchers in Europe.
Scientific Resources
Explore how to optimize Lipid Nanoparticules for mRNA delivery for drug development.
This study highlights key formulation parameters: buffer composition, lipid selection; crucial for enhancing transfection and therapeutic efficacy.
Xavier Warnet PhD, our CRO's project manager published a review on how scientists have developed lipid nanoparticles (LNPs) that make it possible to deliver RNA in a more precise manner, paving the way for future development in oncology.
RNA Therapeutics
Cell Therapy
Biotherapeutic Discovery​