Improve Confidence in Poly(A) Tail Analysis With Defined RNA Reference Standards

Poly(A) tail length is a critical quality attribute of mRNA, affecting RNA stability, translation efficiency and overall product performance. Yet obtaining reliable Poly(A) tail measurements can be challenging when sample heterogeneity and unsuitable reference materials make results difficult to interpret and reproduce.

Accurate Poly(A) tail length determination is essential throughout mRNA analytical development, quality control and method validation. Without well-defined reference materials, comparing results, assessing assay performance or transferring methods between laboratories can become unnecessarily complex.

Key Challenges

Traditional reference materials can introduce variability or require additional preparation and characterisation before they can be integrated into an analytical workflow.

For researchers working with mRNA, this can mean longer development times, less confidence in assay performance and greater difficulty obtaining reproducible, interpretable results.

The Solution

ContrOligo™ Poly(A) RNA Standards from TriLink BioTechnologies provide well-defined, chemically synthesised RNA reference standards with precisely defined Poly(A) tail lengths.

Designed to fit commonly used Poly(A) tail analysis workflows, they provide a straightforward way to support method development, assay qualification, routine testing and method validation.

Key Features

• Chemically synthesised Poly(A) RNA reference standards
• Six defined tail lengths: 40A, 60A, 80A, 100A, 120A and 140A
• High-purity materials with documented QC data
• Available as individual standards or as a multiplex kit
• 10 nmole format suited to HPLC applications
• 2 nmole format suited to mass spectrometry applications

Applications

• mRNA analytical development
• Poly(A) tail length determination
• Assay qualification and validation
• Manufacturing quality control
• Method comparison and technology transfer
• Regulatory and CMC analytical workflows

Case Study

Well-defined reference standards can make it easier to interpret Poly(A) tail analysis results and estimate the tail length of unknown mRNA samples.

The distinct, well-resolved reference peaks provide defined points of comparison across different Poly(A) tail lengths. This can help you evaluate unknown samples, assess analytical performance and build greater confidence in your measurements.

Figure 1: Separation of a mixture of ContrOligos (40A, 60A, 80A, 100A and 120A; blue chromatogram) and RNase T1/A-digested mRNA with an 80A nominal tail (purple) using a reverse-phase method with 100 mM octylammonium acetate. A linear fit curve based on ContrOligo retention times was used to calculate the tail length of the digested mRNA. The calculated tail length was 89A, compared with 84A determined by mass spectrometry. Data provided by TriLink BioTechnologies.

Why Choose ContrOligo™ Poly(A) RNA Standards?

When Poly(A) tail length matters, your reference material matters too.

ContrOligo™ Poly(A) RNA Standards give you defined, characterised reference points across a broad range of tail lengths, helping you develop, evaluate and validate Poly(A) tail analysis methods with greater confidence and reproducibility.

Looking for the right Poly(A) RNA standard for your research? 

Contact our team for help selecting the best ContrOligo™ solution for your Poly(A) tail analysis, method development or quality control workflow.

• References

  Article content created by Tebubio using courtesy materials provided by TriLink Biotechnologies.