Human Brain Microvascular Endothelial Cells (HBMVEC) - 0.5 million cells/vial

Human Brain Microvascular Endothelial Cells (HBMVEC) are the major element of the blood-brain barrier that shields the brain against toxins and immune cells via paracellular, transcellular, transporter, and extracellular matrix proteins [1] . HBMVEC are morphologically different from the peripheral endothelium. Brain endothelial cells lack fenestrations, have minimal pinocytic activity, are connected by tight junctions and have a large number of mitochondria associated with high metabolic activity [2] . Like peripheral endothelial cells, however, HBMVEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. HBMVEC have been widely used for studying the molecular and cellular function of blood-brain barrier [3] .
iXCells Biotechnologies provides high quality HBMVEC, which are isolated from human brain and cryopreserved at P2, with ≥ 0.5 million cells in each vial. These HBMVEC express von Willebrand Factor (vWF), CD31 (PECAM), and Dil-Ac-LDL by uptake. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can be further expanded for no more than 3 passages in Endothelial Cell Growth Media under the conditions suggested by iXCells Biotechnologies. Further expansion may decrease the purity.
Product Details
Organism: Homo Sapiens, Human
Cell Type: Endothelial Cell
Tissue: Human Brain
Disease: Normal
Package Size: 0.5 x 10⁶ cells/vial
Passage Number: P2
Growth Properties: Adherent
Product Format/Shipped: Cryopreserved
Storage: Liquid Nitrogen
Associated Media: Endothelial Cell Growth Media
References
[1] Abbott, N. J., Patabendige, A. A., Dolman, D. E., Yusof, S. R., & Begley, D. J. (2010). Structure and function of the blood-brain barrier. Neurobiology of disease, 37(1), 13–25.
[2] Yang, C., Hawkins, K. E., Doré, S., & Candelario-Jalil, E. (2019). Neuroinflammatory mechanisms of blood-brain barrier damage in ischemic stroke. American journal of physiology. Cell physiology, 316(2), C135–C153.
[3] Godinho-Pereira, J., Garcia, A. R., Figueira, I., Malhó, R., & Brito, M. A. (2021). Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption. International journal of molecular sciences, 22(13), 7057.
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