White DNA Helix

Transform Your Long-Read Sequencing Into a Multiomic Powerhouse

 

CUTANA™ Hia5 is the essential enzyme for Fiber-seq [1], a cutting-edge multiomic long-read sequencing (LRS) assay that simultaneously profiles chromatin accessibility, DNA methylation, and genetic variation at single-molecule resolution.

By layering chromatin accessibility on top of standard LRS workflows, Fiber-seq uniquely links epigenetic information (DNA methylation) and complex genetic architecture, including structural variants and haplotype phasing, to chromatin states within the same DNA molecules (Figure 1).

 

15% off with code FSEQ15 until December 31, 2026

 

Offer non-cumulative with any other discounts, special offers or price agreements.

Figure 1: Overview of the CUTANA Fiber-Seq protocol.

How Fiber-seq Works

CUTANA™ Hia5 is a DNA adenine methyltransferase (MTase) that uses S-adenosylmethionine (SAM) to catalyse methylation at the N6 position of adenine, producing N6-methyladenine (6mA).

In Fiber-seq, Hia5’s nonspecific activity methylates adenines within accessible chromatin regions in situ, effectively recording chromatin accessibility onto native DNA without disturbing endogenous methylation (5mC).

The Hia5-modified DNA is fully compatible with long-read sequencing platforms, including PacBio® HiFi Sequencing and ONT® Nanopore Sequencing, ensuring seamless integration into your existing workflows.

 

Figure 2: Fiber-seq simultaneously detects open chromatin (6mA) and DNA methylation (5mC).

Why CUTANA™ Hia5 Is a Game-Changer

  • Multiomic insights in a single assay
    Consolidate multiple assays into one: Fiber-seq simultaneously profiles chromatin accessibility, DNA methylation, and genetic variants, reducing cost, time, and sample requirements.
  • Highest-activity 6mA-MTase
    Hia5 enables faster labelling and higher-resolution mapping of accessible DNA, with proven applications in protein footprinting, including transcription factor binding and nucleosome positioning [2–4] (refer to Figure 2).
  • Access the hardest-to-sequence regions
    Profile chromatin accessibility in repetitive and structurally complex regions such as transposable elements, centromeres, telomeres, segmental duplications, and chromosomal rearrangements.
  • Easy workflow integration
    Perform Fiber-seq in less than 2 hours, directly upstream of standard long-read sequencing library preparation (illustrated in Figure 1).
  • Resolve chromatin state heterogeneity
    Our PCR-free approach leverages long-read sequencing to profile individual DNA molecules, uncovering heterogeneity in cell populations often missed by short-read methods.

 

CUTANA™ Hia5: Product Specifications

Feature
Details
Type
Adenine Methyltransferase
Molecular Weight
33.8 kDa
Host
E. coli
Epitope Tag
6xHis
Formulation
50 mM Tris pH 8.0, 1 mM DTT, 250 mM NaCl, 10% glycerol

Do you want more information?

Ready to elevate your chromatin and epigenomic research? Fill out the form below to contact us to learn more about CUTANA™ Hia5 and Fiber-seq, and discover how our multiomic solutions can empower your experiments.