Page 2 - Scientific Library

Small GTP-binding proteins such as RhoA, Rac1, and Cdc42 are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology. They bind and hydrolyze

Ras and Rho family members are small G proteins involved in the regulation of actin-dependent cell processes such as motility, growth, and intracellular trafficking. Furthermore, dysfunctions of Ras and

Actin can be stained in living and fixed cells to determine and follow the structure and function of the cytoskeleton. The actin cytoskeleton is a very dynamic and labile structure in the living cell,

Actin serves as one of the major cytoskeleton structures. It is a crucial component involved in a plethora of processes in cell biology:  stabilizing the cell shape, cell movements (e.g. cell migration) 

Actin can exist in two forms: Globular subunit (G-actin) and Filamentous polymer (F-actin). Both forms of actin interact with a plethora of proteins in the cell. To date there are over 50 distinct classes

Actin dynamics - i.e. polymerization of G-actin to form filamentous F-actin or de-polymerization of F-actin

In previous posts,  I invited you to look at versatile methods to

• Visualize actin: Focus on Actin staining and visualization
• Measure

Recently, a we published a series of posts focusing on research

In this post, I invite you to discover some state-of-the art methods to measure the activation of the most prominent small G proteins.

In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins,

You suspect your protein binds to microtubules? That it might stabilize or destabilize these filamentous structures? Then this post is here to help you to find a meaningful assay to validate your assumption.

In a previous post, we looked at the importance of sample preparation