Functional Assays

Von Hippel-Lindau, or VHL, is an E3 Ligase that mediates ubiquitination and proteasomal degradation of target proteins. VHL is indicated to play a large role in tumor suppression, particularly due to its interactions with HIF (hypoxia inducible factor). The VHL is typically used as the required E3 ligase in PROTAC (Proteolysis Targeting Chimera) drug design, protein degradation studies and selectivity profiling. VHL complex  is a multi-subunit ubiquitin ligase constituted with VHL, Elongin B, Elongin C, Cullin-2 and Rbx1. Elongin B (ELOB) and Elongin C (ELOC) as a heterodimer binds to the BC-box motif present in SOCS- & VHL-box protein families. VHL is the substrate recognition component that brings target specificity. VHL is linked to scaffolding proteins Cullin-2/Rbx1 of the complex via adaptor protein Elongin C with Elongin B stabilizing the complex.

Von Hippel-Lindau, or VHL, is an E3 Ligase that mediates ubiquitination and degradation of target proteins. VHL is seen to play a large role in tumor suppression, particularly due to its interactions with HIF. VHL can be used as the required E3 ligase in PROTAC (Proteolysis Targeting Chimera) drug design, protein degradation studies and selectivity profiling.

LifeSensors’ Blocking Concentrate is a proprietary solution blend to allow sensitive plate based assays.

LifeSensors’ sensitive chemiluminescent Detection Reagent 1 & Detection Reagent 2

Decomplexing Buffer designed to be used with PA950.

The Human RGMb / PDL2 Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between programmed cell death 1 ligand 2 and its receptor repulsive guidance molecule B in the presence of potential inhibitors within 4 hours. 4 - 8 week lead time.

The Human TIM3 / Galectin9 Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between galectin-9 and its receptor T-cell immunoglobulin and mucin domain containing 3 in the presence of potential inhibitors within 4 hours. 4 - 8 week lead time.

The Human TIM3 / Galectin9 Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between galectin-9 and its receptor T-cell immunoglobulin and mucin domain containing 3 in the presence of potential inhibitors within 4 hours. 4 - 8 week lead time.