Buffers & Concentrates

The ABTS (2,2?-azino-bis [3-ethylbenzthiazoline-6-sulfonic acid]) substrate is supplied as a one component, ready-to-use solution for ELISA applications. A soluble blue-green reaction product is obtained when this substrate system is reacted with horseradish peroxidase.

BioFX? Alkaline Phosphatase/Horseradish Peroxidase Block is supplied as a ready-to-use blocking reagent for immunohistochemistry samples. The solution blocks both alkaline phosphatase and peroxidase endogenous enzymatic activity.

Absorbance is read in the yellow spectral range of 405 nm - 420 nm.

Absorbance is read in the yellow spectral range of 405 nm - 420 nm.

Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and membrane assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the dioxetane substrate, antibody/antigen titers and blockers. Surmodics provides multiple 1-component AP chemiluminescent substrates with various intensities and wavelength emissions to allow the assay developer to choose the appropropriate substrate for the application. Features: Absorbance at 450 nm. High signal-to-noise ratios.

The substrate is a ready to use, one-component formluation of 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium for use in immunblotting or other membrane applications.

The substrate is a ready to use, one-component formluation of 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium for use in immunblotting or other membrane applications.

The substrate is a ready to use, one-component formluation of 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium for use in immunblotting or other membrane applications.

The substrate is a ready to use, one-component formluation of 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium for use in immunblotting or other membrane applications.