Labelling

SiR-actin is based on the F-actin binding natural product jasplakinolide. SiR-actin is fluorogenic, cell permeable and highly specific for F-actin. Sir-actin stains endogenous F-actin without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-actin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.

-High sensitivity for intracellular Fe2+ detection -Suitable for live cell imaging -Applicable for plate reader assay

- Selective measurement of Lipid Peroxide - Less cellular photo-damage - Applicable for microscopy and FCM analysis

Fluorescent Probe for Mitochondrial Research - Cell membrane permeable probe - Mitochondria targeted imaging is possible - Desirable fluorescent properties (ex. 452nm / em. 47 nm)

-High sensitivity for Fe2+ detection in mitochondria -Suitable for live cell imaging