Secondary Antibodies

Solution 1.2 mg/ml in the buffer (0.1 M sodium chloride, 0.01M sodium phosphate pH 7.5, 50% glycerol, 5 mg/ml BSA). Specific to human IgM (heavy chain). No cross-reactivity towards human IgG, IgA and other human plasma proteins

Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Rabbit IgG monoclonal secondary antibody. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional HRP Anti-Rabbit IgG blotting reagent with Rabbit IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. Rabbit IgG HRP TrueBlot has been tested in ELISA, western blot, and immunoprecipitation and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.

Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag is affinity purified and specific for rabbit immunoglobulins. Minimal cross reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope [1]. For best results, use with CUTANA™ pAG-Tn5 (EpiCypher 15-1017 & 15-1117). For detailed instructions for use, see EpiCypher’s CUTANA™ CUT&Tag protocol: epicypher.com/protocols. *Mixed Monoclonal: a pool of multiple monoclonal antibodies.