Results for ELISA Kits ( 63884 )
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-6 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-6 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
The Biosensis Cystatin C Rapid™ enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of Cystatin C in less than 4 hours in human saliva only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the kit protocol for specific use instructions for each substrate application. This kit consists of a pre-coated polyclonal rabbit anti-human Cystatin C capture antibody, a biotinylated goat anti-human Cystatin C detection antibody, a recombinant human Cystatin C standard, and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Cystatin C present in samples and protein standards. A Cystatin C positive control (QC sample) is provided to assure consistent assay performance. This kit has not been tested for other applications. It has been configur
The Biosensis Human IL-1RA Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of interleukin-1 receptor antagonist (IL-1RA) in less than 4 hours in human saliva only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the kit protocol for specific use instructions for each substrate application. This kit consists of a pre-coated polyclonal rabbit anti-human IL-1RA capture antibody, a biotinylated goat anti-human IL-1RA detection antibody, a recombinant human IL-1RA standard, and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of IL-1RA present in samples and protein standards. An IL-1RA positive control (QC sample) is provided to assure consistent assay performance. This kit has not been tested for other applications. It has