Enzymes

Recombinant human AMPK (combination of A1/B1/G3 subunits), full length, with C-terminal His-tags, expressed in Sf9 insect cells via a baculovirus expression system. MW = 68 kDa (A1), 38 kDa (B1), 51 kDa (G3).

Recombinant human AMPK (combination of A1/B2/G1 subunits), full length, with C-terminal His-tags, expressed in Sf9 insect cells via a baculovirus expression system. MW = 68 kDa (A1), 36 kDa (B2), 40 kDa (G1).

Recombinant human AMPK (combination of A2/B1/G1 subunits), full length, with C-terminal His-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. The gene accession number for the three subunits (A1/B1/G1) is NM_006251, NM_006253, and NM_002733, respectively. This protein has been activated with CAMKK.

Recombinant human AMPK (combination of A2/B2/G1 subunits), full length, with C-terminal His-tags, expressed in Sf9 insect cells via a baculovirus expression system. MW = 69 kDa (A2), 36 kDa (B2), 41 kDa (G1).

Recombinant human AMPK (combination of A2/B2/G2 subunits), full length, with C-terminal His-tags, expressed in Sf9 insect cells via a baculovirus expression system. MW = 69 kDa (A2), 36 kDa (B2), 65 kDa (G2).

Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_005923.

Recombinant human CAMK4, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Recombinant mouse CAMK1, full length, with N-terminal GST-tag, expressed in E. coli cells