Enzymes

Human transmembrane leukocyte antigen related PTP (LAR), GenBank accession No. NM_130440, a.a. 1330-1620 (catalytic domain) with N-terminal GST-tag, MW=58 kDa, expressed in an E. coli expression system.

Protein tyrosine phosphatase PTPRG, also known as PTPγ (gamma) (GenBank accession No. NM_002841), a.a. 801-1147 (catalytic domain) with N-terminal GST-tag, MW=65.6 kDa, expressed in an E. coli expression system.

Protein tyrosine phosphatase DEP1 (density-enhanced phosphatase-1), also known as PTPRJ, PTP-eta and CD148 (GenBank accession no. NM_U10886), a.a. 1024-1306 (catalytic domain) with N-terminal GST-tag, MW=58.9 kDa, expressed in an E. coli expression system.

PTPµ (mu) (protein tyrosine phosphatase mu, also known as RPTPµ), catalytic domain, (a.a. 896-1175), (GenBank Accession No. NM_001105244) with N-terminal GST tag, MW=56.2 kDa, expressed in an E. coli expression system.

Pseudophosphatase PTP-IA2, also known as PTPRN and ICA-512, GenBank accession no. NM_002846, a.a. 693- 979, with an N-terminal GST-tag, MW=58.8 kDa, expressed in an E. coli expression system.

Protein complex of human protein phosphatase 2A catalytic subunit ? (PP2A?), also known as TPD3, RP-C, PP2Ac, and PPP2CA (GenBank Accession NM_002715, a.a. 2-309 with C-terminal Histag) and human protein phosphatase 2A regulatory subunit (PP2AR) (GenBank Accession NM_014225, a.a. 2-589 with Nterminal GST-tag). Co-expressed in a baculovirus-infected Sf9 cell expression system. MW = 36 kDa (PP2A) and 92 kDa (PP2AR).

Human Akt2 (GenBank Accession No. NM_001626), full length with N-terminal His-tag, MW=57 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human Aurora kinase B (GenBank Accession No. NM_004217), a.a. 2-344, with N-terminal GST-tag and C-terminal His-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human AKT Serine/Threonine Kinase 1 (AKT1), also known as Protein Kinase B Alpha (PKBα), and RAC, GenBank Accession No. NM_001014432, a.a. 1-480(full length) with N-terminal His-tag, was expressed in Sf9 insect cells via a baculovirus expression system. MW=60 kDa. The purified AKT1 is activated in vitro using PDK1 enzyme.