Functional Assays

The Thrombin Inhibitor Screening Assay is a colorimetric assay designed to measure the activity of human alpha thrombin for screening and profiling applications. The assay kit comes in a convenient 96-well or 384-well format and contains enough purified human alpha thrombin, a chromogenic substrate, and PR-02 buffer for 100 or 400 reactions. To determine the effect of an inhibitor on Thrombin activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the chromogenic substrate to the reaction. The assay was functionally validated using Dabigatran, a potent inhibitor of thrombin. Upon proteolysis, thrombin cleaves the chromogenic substrate at the C-terminal end releasing p-nitroanilid (pNA), which produces a yellow color that is measurable photometrically at λ=405 nm. The increase in color is proportional to thrombin activity.

The Thrombin Inhibitor Screening Assay is a colorimetric assay designed to measure the activity of human alpha thrombin for screening and profiling applications. The assay kit comes in a convenient 96-well or 384-well format and contains enough purified human alpha thrombin, a chromogenic substrate, and PR-02 buffer for 100 or 400 reactions. To determine the effect of an inhibitor on Thrombin activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the chromogenic substrate to the reaction. The assay was functionally validated using Dabigatran, a potent inhibitor of thrombin. Upon proteolysis, thrombin cleaves the chromogenic substrate at the C-terminal end releasing p-nitroanilid (pNA), which produces a yellow color that is measurable photometrically at λ=405 nm. The increase in color is proportional to thrombin activity.

The Factor Xa Inhibitor Screening Assay is a colorimetric assay designed to measure the activity of human Factor Xa for screening and profiling applications. The assay kit comes in a convenient 96-well format or 384-well format and contains enough purified human Factor Xa, a chromogenic substrate, and PR-02 buffer for 100 or 400 reactions. To determine the effect of an inhibitor on Factor Xa activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the chromogenic substrate to the reaction. The assay was functionally validated using Rivaroxaban, a potent inhibitor of Factor Xa. Upon proteolysis, Factor Xa cleaves the chromogenic substrate at the C-terminal end releasing p-nitroanilid (pNA), which produces a yellow color that is measurable photometrically at λ=405 nm. The increase in color is proportional to Factor Xa activity.

The Factor Xa Inhibitor Screening Assay is a colorimetric assay designed to measure the activity of human Factor Xa for screening and profiling applications. The assay kit comes in a convenient 96-well format or 384-well format and contains enough purified human Factor Xa, a chromogenic substrate, and PR-02 buffer for 100 or 400 reactions. To determine the effect of an inhibitor on Factor Xa activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the chromogenic substrate to the reaction. The assay was functionally validated using Rivaroxaban, a potent inhibitor of Factor Xa. Upon proteolysis, Factor Xa cleaves the chromogenic substrate at the C-terminal end releasing p-nitroanilid (pNA), which produces a yellow color that is measurable photometrically at λ=405 nm. The increase in color is proportional to Factor Xa activity.

The IDE (Insulin-degrading enzyme) Inhibitor Screening Assay is a homogeneous fluorogenic assay designed to measure the activity of IDE for screening and profiling applications. The IDE assay kit comes in a convenient 96-well or 384-well format and contains enough purified IDE (amino acids 71-254), substrate, and PR-02 buffer for 100 or 400 reactions.

The IDE (Insulin-degrading enzyme) Inhibitor Screening Assay is a homogeneous fluorogenic assay designed to measure the activity of IDE for screening and profiling applications. The IDE assay kit comes in a convenient 96-well or 384-well format and contains enough purified IDE (amino acids 71-254), substrate, and PR-02 buffer for 100 or 400 reactions.

The Chemi-Verse™ CDK3/CyclinE1 Kinase Assay is a luminescent assay designed to measure CDK3 (cyclin-dependent kinase 3)/CyclinE1 kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant complex of CDK3/CyclinE1, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ CDK17/Cyclin Y Kinase Assay is a luminescent assay designed to measure CDK17 (cyclin-dependent kinase 17)/Cyclin Y kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant complex of CDK17/Cyclin Y, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ CDK8/Cyclin C Kinase Assay is a luminescent assay designed to measure CDK8 (cyclin-dependent kinase 8)/Cyclin C kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant complex of CDK8/Cyclin C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.