DNA/RNA preparation Kits

The RNA Labeling Kit produces alkyne-modified RNA by in vitro transcription from bacteriophage T7 RNA polymerase promoters. The kit utilizes 5-Ethynyl-UTP (5-EUTP) nucleotide to firstly incorporate alkynes into RNA. The resulting RNA contains alkyne modified Uracil, which subsequently react with azides of e.g. fluorescent dyes, haptenes and others in a highly selective fashion under benign click reaction conditions. One advantage is that the label incorporation can be tuned by the UTP to 5-EUTP ratio.

The RNA Labeling Kit produces alkyne-modified RNA by in vitro transcription from bacteriophage T7 RNA polymerase promoters. The kit utilizes 5-Ethynyl-UTP (5-EUTP) nucleotide to firstly incorporate alkynes into RNA. The resulting RNA contains alkyne modified Uracil, which subsequently react with azides of e.g. fluorescent dyes, haptenes and others in a highly selective fashion under benign click reaction conditions. One advantage is that the label incorporation can be tuned by the UTP to 5-EUTP ratio.

The E-Z 96™ FastFilter Plasmid Kit is optimized for the parallel preparation of up to 96 plasmid minipreps in 1 hour. The Lysate Clearance Plate replaces the time-consuming centrifugation involved in clearing of bacterial alkaline lysates. This kit contains all of the components needed for plasmid DNA purification in a 96-well format. Purified plasmid DNA is suitable for the most sensitive downstream applications, such as routine screening or automated DNA sequencing. The E-Z 96™ Fastfilter Plasmid Kits newly designed 96-well DNA plates are ideal for use with automated liquid handlers.

"The CUTANA™ Quick Cleanup DNA Purification Kit can be used to purify CUT&RUN or CUT&Tag DNA and remove unwanted adapter-/primer-dimers from these reactions. Unlike many kits that purify DNA using spin columns, this kit uses SPRI paramagnetic beads for streamlined, higher throughput workflows easily integrated into CUTANA™ genomic mapping assays. Depending on the application, various SPRI bead:DNA ratios are added to capture target DNA. In CUT&RUN, pAG-MNase cleaves DNA proximal to antibody-labeled chromatin, releasing it into the supernatant. It is imperative to recover the highest DNA yield possible from the supernatant; therefore, a higher bead:DNA ratio is added to span a wide range of DNA fragment lengths. This kit includes sufficient volume to perform 48 total CUT&RUN reactions at an empirically optimized higher bead ratio. In CUT&Tag, pAG-Tn5 cleaves antibody-bound chromatin and simultaneously ligates adapter DNA, resulting in longer DNA fragments. Further, in the EpiCypher C