Assays & Kits

The BLM helicase, also known as Bloom syndrome protein, is a key enzyme involved in DNA replication and repair (DDR). The BLM helicase is a member of the RecQ family of helicases, which are evolutionarily conserved and found in many organisms, including bacteria, yeast, and humans. It catalyzes the unwinding of duplex DNA with 3' to 5' directionality, driven by the energy generated from ATP hydrolysis. BLM plays a crucial role in maintaining genomic stability by unwinding DNA structures during processes such as DNA replication, recombination, and repair. Mutations in the BLM gene can lead to Bloom syndrome, a rare genetic disorder characterized by growth deficiency, sun-sensitive skin lesions, and an increased risk of cancer. High expression of BLM is found in glioblastoma, and it was found that inhibition of its activity leads to increased susceptibility to treatment with drugs targeting other proteins involved in DDR, such as PARP1 (poly-ADP ribosylation protein 1). The use of BLM in

"The CUTANA™ Quick Cleanup DNA Purification Kit can be used to purify CUT&RUN or CUT&Tag DNA and remove unwanted adapter-/primer-dimers from these reactions. Unlike many kits that purify DNA using spin columns, this kit uses SPRI paramagnetic beads for streamlined, higher throughput workflows easily integrated into CUTANA™ genomic mapping assays. Depending on the application, various SPRI bead:DNA ratios are added to capture target DNA. In CUT&RUN, pAG-MNase cleaves DNA proximal to antibody-labeled chromatin, releasing it into the supernatant. It is imperative to recover the highest DNA yield possible from the supernatant; therefore, a higher bead:DNA ratio is added to span a wide range of DNA fragment lengths. This kit includes sufficient volume to perform 48 total CUT&RUN reactions at an empirically optimized higher bead ratio. In CUT&Tag, pAG-Tn5 cleaves antibody-bound chromatin and simultaneously ligates adapter DNA, resulting in longer DNA fragments. Further, in the EpiCypher C

Cytoplasmic isocitrate dehydrogenase (ICDHc, EC 1.1.1.42) is widely distributed in animals, plants, microorganisms, and cultured cells. It catalyzes the decarboxylation and dehydrogenation of isocitrate to produce α-ketoglutarate, while simultaneously reducing NADP+ to NADPH. ICDHc serves as another significant source of NADPH in the cytoplasm, apart from the pentose phosphate pathway, and its activity often undergoes notable changes under stress conditions. CheKine™ Micro Isocitrate Dehydrogenase Cytoplasmic (ICDHc) Activity Assay Kit is capable of detecting cytosolic isocitrate dehydrogenase (ICDHc) activity in animal and plant tissues, cells, or bacteria, as well as in serum (plasma). The principle behind this involves utilizing ICDHc's catalytic action in reducing NADP+ to NADPH, with the subsequent increase in NADPH concentration being measured at a wavelength of 340 nm.

β-glucuronidase (β-GD), a matrix degradation enzyme involved in tumor invasion and metastasis, is widely found in animal tissues. It has physiological functions such as hydrolyzing sterol glucuronic acid and acidic mucopolysaccharide. The content of this enzyme is high in hepatocytes. In addition, it is rich in gastric cancer, so the determination of β-GD activity in gastric juice is of great significance for the study of gastric cancer. CheKine™ Micro β-glucuronidase (β-GD) Activity Assay Kit can detect animal tissues. In this kit, β-GD catalyzed phenol β-D-glucuronic acid to produce free phenolphthalein, and the enzyme activity was determined by determining the content of phenol.

Fructose-1,6-diphosphate (FDP) is an important intermediate product in the glycolysis process. It can regulate a variety of enzymes, improve cell energy metabolism, increase energy utilization, anti-arrhythmia and anti-tissue peroxidation. FDP is widely used in clinical medicine. CheKine™ Micro Fructose-1,6 Diphosphate (FDP) Content Assay Kit can be used to detect biological samples such as animal tissue, bacteria or cells, serum or plasma. In the kit, aldolase catalyzes the cleavage of fructose 1,6-diphosphate. The product reacts with 2,4- dinitrophenylhydrazine in acid medium to form 2, 4-dinitrophenylhydrazone, which is dark red in alkaline solution and has a characteristic absorption peak at 540 nm.

The activity of plant dehydrogenase (PDHA) is largely reflects the active state of the organism, which can directly indicate the ability of biological cells to degrade its matrix. CheKine™ Micro Plant Dehydrogenase (PDHA) Activity Assay Kit can be used to detect biological samples such as plant tissues. In this kit, The hydrogen acceptor 2,3,5-triphenyl tetrazolium chloride (TTC) generates red triphenyl formazone (TFF) after receiving hydrogen during cell respiration. TFF has a characteristic absorptionpeak at 485 nm, the PDHA activity can quantified by measuring the absorbance at 485 nm.

Asparagine synthetase (AS) is a widely distributed enzyme in living organisms belonging to the class of amino transferases. It catalyzes the transfer of an amine group from glutamine to aspartic acid. When a plant is subjected to ammonia toxicity, the formation of asparagine serves as a detoxification mechanism. CheKine™ Micro Asparagine Synthetase (AS) Activity Assay Kit is designed to quantify asparagine synthetase activity in animal and plant tissues, bacterial and cellular samples, as well as in serum (plasma). The assay principle relies on the AS enzyme's ability to catalyze the hydrolysis of L-asparagine into L-aspartic acid and ammonia. By employing Nessler's reagent to detect the rate of ammonia accumulation, the enzymatic activity of AS can be determined.

Nitrate reductase (NR, EC1.7.1.3) exists widely in plants. It is not only the key enzyme for the conversion of nitrate nitrogen to ammonia nitrogen, but also an inducing enzyme, which has an effect on the yield and quality of crops. CheKine™ Micro Nitrate Reductase (NR) Activity Assay Kit allows for the detection of nitrate reductase activity in plant tissues, bacterial, or cellular samples. The principle behind this assay involves NR catalyzing the reduction of nitrate to nitrite, following the reaction:NO3ˉ+NADH+H＋ →NO2ˉ+NAD＋ +H2O. The resulting nitrite, under acidic conditions, then reacts quantitatively with p-amino benzenesulfonic acid and α-naphthylamine to produce a red azo compound. This red azo compound exhibits a maximum absorption peak at 540 nm, which can be measured by spectrophotometry, thereby enabling the quantification of nitrate reductase activity in the sample.

Nitrite reductase (NiR) is a crucial enzyme in the reduction process of nitrite nitrogen and plays a vital role in the nitrogen cycle of nature. It is widely distributed in microorganisms and plants, capable of catalyzing the reduction of nitrite, thereby decreasing the accumulation of nitrite nitrogen in the environment and alleviating its toxic effects on the growth and development of organisms. CheKine™ Micro Nitrite Reductase (NiR) Activity Assay Kit is designed to quantify nitrite reductase activity in plant tissues, bacterial, and fungal samples. It operates on the principle that nitrite reductase catalyzes the reduction of NO2- to NO, thereby reducing the amount of NO2- available in the sample to participate in diazotization reactions that form a purple-colored compound. The change in absorbance at 540 nm reflects the activity of NiR in the sample.