Labelling

SPY650-DNA is a bright, photostable, far-red & non toxic live cell nuclear stain based on our SPYTM dyes series. Its optimized structure allows quick labeling of DNA in live & fixed cells with high specificity and very low background. SPY650-DNA stains the nuclei of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SPY650-DNA enables multicolor imaging with SPY505, SPY555, SPY595, SPY700, GFP or m-cherry. SPY650-DNA can be imaged with standard Cy5 filtersets. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Probe quantity allows 100 staining experiments.

SPY650-FastAct™ is a bright, far red, fluorogenic & non toxic F-actin stain based on SPY™ dyes series. Its optimized structure allows to label F-actin in live cells with high specificity and low background. The unique and unmatched feature of SPY650-FastAct™ is its ability to label very dynamic actin, like LifeAct™* but the probe does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. SPY650-FastAct™ is based on our bright, photostable & far red SPY650 fluorophore which is far superior than fluorescent proteins. SPY650-FastAct™ enables multicolor imaging with SPY505, SPY555, SPY595, SPY700, GFP or m-cherry. SPY650-FastAct™ can be imaged with standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging of living cells and tissue.*LifeAct™ is a registered trademark of Ibidi GmbH.

SiR-lysosome is based on the cathepsin D binding natural product pepstatin A. SiR-lysosome is fluorogenic, cell permeable and highly specific for lysosomes. Sir-lysosome stains lysosomes in ilve cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-lysosome is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-lysosome can be used for widefield, confocal, SIM or STED imaging in living cells and tissue.

SiR700-actin is based on the F-actin binding natural product jasplakinolide. SiR700-actin is fluorogenic, cell permeable and highly specific for F-actin. SiR700-actin stains endogenous F-actin without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-actin is compatible with GFP and/or m-cherry fluorescent proteins. SiR700-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue.

SiR700-tubulin is based on the microtubule binding drug docetaxel. SiR700-tubulin is fluorogenic, cell permeable and highly specific for microtubules. SiR700-tubulin stains endogenous microtubules without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-tubulin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR700-tubulin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue.

SiR700-DNA is based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR700-DNA is fluorogenic, cell permeable and highly specific for DNA. SiR700-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-DNA is compatible with GFP and/or m-cherry fluorescent proteins and is a non cytotoxic alternative to DRAQ5. It can be imaged with standard Cy5 filtersets. SiR700-DNA can be used for widefield, confocal, SIM or STED imaging in living cells and tissue

SiR700-lysosome is based on the cathepsin D binding natural product pepstatin A. SiR700-lysosome is fluorogenic, cell permeable and highly specific for lysosomes. SiR700-lysosome stains lysosomes in ilve cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-lysosome is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR700-lysosome can be used for widefield, confocal, SIM or STED imaging in living cells and tissue.

ER Flipper-TR™ is a fluorescent probe that specifically targets the endoplasmic reticulum (ER) of cells and reports membrane tension changes through its fluorescence lifetime changes. It contains an ER targeting motive as well as a Flipper fluorophore which senses changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. ER Flipper-TR™ spontaneously labels the ER of cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm.

Lyso Flipper-TR is a fluorescent probe that specifically targets the membrane of lysosomes of cells and reports membrane tension changes through its fluorescence lifetime changes. It contains a lysosome targeting motive as well as a Flipper fluorophore which senses changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. Lyso Flipper-TR spontaneously labels lysosomes of cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm.