PCR & RT Kits

For post-transcriptional poly(A) tail addition to RNAs. Allows tailing of mRNAs in vitro, to lengths equivalent to or greater than those found in nature. This cannot be accomplished with co-transcriptional tailing methods.

Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers (for use with miRNA qPCR primers).

For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in 30 minutes.

All-in-One™ miRNA qRT-PCR Detection Kit 2.0* (20 RT and 200 qPCR reactions) (Old cat # QP015)

Simply the best way to make in vitro mRNA. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. See T7-FlashScribe Transcription Kit, ScriptCap m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page.

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods.