CUTANA CUT&Tag Kit with Primer Set 1 Subset - 24 Reactions

The CUTANA™ CUT&Tag Kit offers a comprehensive solution for ultra-sensitive mapping of histone post-translational modifications (PTMs). In CUT&Tag, antibody-bound chromatin is selectively cleaved with fusion protein pAG-Tn5, which simultaneously adds sequencing adapters. Tagmented fragments bypass traditional library prep with CUTANA™ CUT&Tag Kit’s exclusive Direct-to-PCR strategy, enabling users to go from cells to PCR-amplified sequencing libraries in one tube with minimal sample loss. The recommended input for CUT&Tag is 100,000 native nuclei per reaction. Comparable data can be generated down to 10,000 nuclei, and the protocol is also validated for whole cells, cryopreserved samples, and lightly cross-linked nuclei or cells. CUT&Tag provides robust profiling for histone PTMs; for chromatin-associated proteins (e.g., transcription factors), CUTANA™ CUT&RUN is recommended (EpiCypher 14-1048, EpiCypher 14-1001). The CUT&Tag Kit Version 4 (v4) now includes two new Wash Buffer Enhance