Stem cell-derivative Motor Neurons / Muscle fiber co-culture functional bio-assay

Human Induced Pluripotent (HIP™) Neural cells

Chipman et al. (Dalhousie University, Halifax, Nova Scotia, Canada) have developed a new cell-based system “mimicking” in vitro Mature Neuromuscular Junction (NMJs). (1)

Chipman et al. PLOSOne (2014) DOI: 10.1371/journal.pone.0091643
Chipman et al. PLOSOne (2014) DOI: 10.1371/journal.pone.0091643

NMJs play an important role in the functionality of Motor neurons.

Their dysfunction is seen in the early stages of various Motor Neuron Diseases (MNDs) like the Amyotrophic Lateral Sclerosis (ALS), Spinal Muscular Atrophy (SMA), Primary Lateral Sclerosis (PLS), Progressive Muscular Atrophy (PMA)…

To meet these needs, Chipman et al. have created a clever co-culture system using Embryonic Stem Cell-derived Motor Neurons (ESCMNs) with primary myotubes. They also reported that NCAM-/- ESCMN/myofiber co-cultures exhibit the same phenotypes observed in NCAM-/- mice.

This work give hopes to study MNDs in vitro by using motor neurons differentiated from induced Pluripotent Stem cells (iPS) derived from individuals with ALS and SMA.

Want to know more about cell-based assays and co-culture systems?

(1) Chipman et al. “A Stem-Cell Based Bioassay to Critically Assess the Pathology of Dysfunctional Neuromuscular Junctions” (2014) PLOSone, March 13. DOI: 10.1371/journal.pone.0091643

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