SIRT1 is a NAD dependent class III Histone Deacetylase (HDAC). The tumor suppressor p53 is not the unique SIRT1 substrate. SIRT1 also deacetylates various key protein targets (PTEN, FOX, HIF-1, XPA, SMAD7 …) involved in the regulation of cellular survival, proliferation and angiogenesis.
SIRT1 – an attractive druggable target in oncology
SIRT1 has rapidly been considered a valuable druggable target because of its implication in p53-dependent apoptosis in response to cellular stress and DNA damage. This enthusiasm was even more enhanced in 2011 when certain studies suggested that a natural dietary polyphenolic compound (Resveratrol) could promote healthy ageing and increase mouse and yeast life span through activation of sirtuins. (1)
Today, SIRT1 returns to the front of the stage with its possible involvement in cellular reprogramming and pluripotency.
SIRT1 – an attractive candidate in regenerative medicine
De Bonis at al. have recently shown the role of SIRT1 as a modulator of cell pluripotency and differentiation. (2)
The authors showed that SIRT1, by interacting with c-myc, is necessary for telomere elongation, and influences telomeric structure and transcription after cell reprogramming. They confirmed that SIRT1 is required for genomic stability of iPSCs and is involved in the maintenance of “good-quality” iPSCs.
Due to its role in chromatin remodeling, gene expression regulation and genomic stability, SIRT1 is again a source of inspiration for researchers in drug discovery, cancer research and cellular reprogramming.
Sources:
(1) Fernández A.F.et al. “The effects of the dietary polyphenol resveratrol on human healthy aging and lifespan” (2011) Epigenetics, Vol. 6 Issue 7, 870-8. DOI: 10.4161/epi.6.7.16499.
(2) De Bonis M.L. et al. “SIRT1 Is Necessary for Proficient Telomere Elongation and Genomic Stability of Induced Pluripotent Stem Cells” (2014) Stem Cell Reports, Vol. 2, 1–17. DOI: 10.1016/j.stemcr.2014.03.002
Looking for reliable SIRT1 research reagents for Drug discovery and Stem cell research?
- SIRT1 activity measureament assays (fluorogenic) by BPS Bioscience
- SIRT1 small molecule inhibitors and activators (including Resveratrol) by Focus Biomolecules
- SIRT1 Human promoter report assays (eGFP, mCHERRY, Puro…) by GeneCopoeia
- Anti P-Sir2/SIRT1 (phospho Ser47) by GeneTex validated for ICC, IF, IHC (P), WB
- ChIP-grade Anti-SIRT1 by Abnova
- and much more SIRT1-based reagents here!
