Quick & easy cellular senescence tools!

Research into cellular senescence is very important for understanding the intracellular functions that control cell survival and death.

Discover these four types of kits and reagents that can you can select according to your evaluation method and purpose of cell senescence.

Product	Detection Kit – SPiDER-BGal	Plate Assay Kit SPiDER-BGal	Cell Cycle Assay Solution Deep Red/Blue	Nucleolus Bright Green/Red
Item#	SG03-10	SG05-01, SG05-05	Deep Red: C548-10, Blue: C549-10	Green: N511-10, Red: N512-10
Best for	In case of difficulty quantifying data or performing multiple staining with X-gal	For processing multiple samples and evaluating senescent cells 1st time	For evaluation using indicators other than SA-B-Gal	For evaluation using indicators other than SA-B-Gal
Detection	Fluorescence	Fluorescence	Fluorescence	Fluorescence
Wavelength (Ex/Em)	Ex. 500 – 540 nm / Em. 530 – 570 nm	Ex. 535 nm / Em. 580 nm	Deep Red: Ex. 633-647 nm / Em. 780/60 nm Blue: Ex. 405-407 nm / Em. 450/50 nm	Green: Ex. 513 nm / Em. 538 nm Red: Ex. 537 nm / Em. 605 nm
Instrument	Fluorescence microscope, FCM	Fluorescence microplate reader	FCM	Fluorescence microscope
Sample	Live cells, fixed cells	Live cells	Live cells, fixed cells	Fixed cells
Target	SA-B-gal activity	SA-B-gal activity	Nucleus	Changes in the nucleolus
Detection Method	Imaging, Substrate: SPiDER-BGal	Plate assay, Substrate: SPiDER-BGal	Flow cytometry	Imaging Detection of the nucleolus by RNA-staining reagent

Webinar
Cellular senescence studies
and analysis methods

References

González-Gualda E, Baker AG, Fruk L, Muñoz-Espín D. A guide to assessing cellular senescence in vitro and in vivo – PubMed (nih.gov). FEBS J. 2021;288(1):56-80. doi:10.1111/febs.15570.
Amor C, Feucht J, Leibold J, et al. Senolytic CAR T cells reverse senescence-associated pathologies – PubMed (nih.gov). Nature. 2020;583(7814):127-132. doi:10.1038/s41586-020-2403-9.
Johmura Y, Yamanaka T, Omori S, et al. Senolysis by glutaminolysis inhibition ameliorates various age-associated disorders – PubMed (nih.gov). Science. 2021;371(6526):265-270. doi:10.1126/science.abb5916.

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