City-Mix™ represents a transformative advancement in cell culture technology. This patented, non-toxic formulation offers a range of exclusive serum alternative supplements that facilitate cell proliferation and extracellular matrix production, all while being animal-free.
The ethical and sustainable profile of City-Mix™, coupled with its ability to reduce batch-to-batch variability, provides substantial cost savings and enhances the efficiency and effectiveness of cell culture media in laboratory settings. Supplied as a 25× liquid concentrate, City-Mix™ allows for fast preparation and easy dilution into any media, making it a conscientious choice for a wide array of biotech applications.
City-mix™ is shown to increase immortalised murine proliferation in simple serum free media formulations.
Immortalised murine myoblasts (left) and murine pre-adipocytes (right) were grown in culture for a 3-day period. Cell number was assessed using alamarBlue™ cell viability assay and expressed as the mean ± S.D. Significance was assessed by an unpaired T-Test.
City-mix™ shown to increase proliferation of Immortalised and primary porcine cells in reduced supplemented serum free media.
Immortalised porcine myoblasts (left), primary porcine pre-adipocyte (centre) and primary porcine fibroblasts (right) were grown in culture for a 7-day period. Cell number was assessed using nuclei staining and expressed and expressed as the mean ± S.D.
City-mix™ is shown to facilitate human dermal fibroblast tissue production via extracellular matrix establishment.
Primary human dermal fibroblasts in culture for a 14-day period. Total collagen I, collagen III, elastin and decorin were assessed using quantitative immunofluorescence analysis utilising Odyssey Infrared imager (LiCor) and expressed as the mean ± S.D. Significance was assessed by an unpaired T-Test.
City-mix™ is a range of proprietary media supplements specially designed to boost cell growth and tissue production in a wide variety of culture conditions. City-mix™ is a chemically-defined solution comprising highly stable and inert, food safe, ingredients that enhance the chemical activity of most components found in common culture media (e.g., albumin, growth factors, nutrients, enzymes, etc) via volume exclusion mechanisms.
City-mix™ is not a complete cocktail of growth factors, but works by reducing or eliminating growth factor supplementation in culture media.
City-mix™ effectively increases proliferation rates and/or extracellular matrix establishment across all the cell lines we have tested it on. However, certain cells perform better with different City-mix™ components. 3D Bio-tissues have optimised the formulations across multiple cell lines which has resulted in different City-mix™ products.
Shanghai-Mix™ – the most versatile cell booster for enhancing cell and tissue growth, effective in both serum-free or serum-containing media.
Myoblasts, adipocytes, fibroblasts
Singapore-Mix™ – Another good all-rounder, particularly effective for enhanced fat cell
growth and for increased production of connective tissue.
Adipocytes, myoblasts, fibroblasts
Tokyo-Mix™ – Low viscosity booster, and particularly effective for enhanced stem cell proliferation
ASC/PSCs
London-Mix™ – The best supplement for enhanced extracellular matrix production.
Fibroblasts, myoblasts, adipocytes
Miami-Mix™ – A cell booster useful to control spontaneous cell differentiation occurring in serum-free conditions
City-mix™ is not a direct serum replacement, and does not contain the nutrients and growth factors commonly found in animal serum. Media supplemented with City-mix™ will still need to include specific growth factors to be effective, but at much lower concentrations than typically used.
City-mix™ can effectively reduce the amount of many of the essential growth factors necessary to sustain specific culture processes such as cell proliferation and tissue maturation. City-mix™ will make these expensive media ingredients go much further via volume exclusion effects. In particular, using City-mix™ to reduce albumin supplementation allows for chemically-defined, xeno-free medium formulations to become cost-effective.
Yes, by enhancing the activity and bio-availability of essential media ingredients such as albumin and growth factors, City-Mix™ allows using less of these ingredients while achieving the same cell performance. Try using City-Mix™ in serum-free media with reduced levels of growth factor supplementation (i.e. between half and one-fifth of normal growth factor concentrations).
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