Lentivirus

Firefly luciferase has been used as a sensitive reporter to study a wide range of biological responses. mCherry is a monomeric red fluorescent protein derived from DsRed found in the sea anemones Discosoma. It belongs to the mFruit family of monomeric red fluorescent proteins, which are improved versions of mRFP1 (monomeric red fluorescent protein 1) in terms of brightness and photostability. The use of fluorescent proteins allows for direct visualization of transfected or transduced cells under a fluorescent microscope or analysis by flow cytometry. The use of lentiviruses to introduce both luciferase and mCherry is a convenient strategy that allows expression of the markers in almost all mammalian cells and to easily determine transduction efficiency and access cellular responses.

Firefly luciferase has been used as a sensitive reporter to study a wide range of biological responses. mCherry is a monomeric red fluorescent protein derived from DsRed found in the sea anemones Discosoma. It belongs to the mFruit family of monomeric red fluorescent proteins, which are improved versions of mRFP1 (monomeric red fluorescent protein 1) in terms of brightness and photostability. The use of fluorescent proteins allows for direct visualization of transfected or transduced cells under a fluorescent microscope or analysis by flow cytometry. The use of lentiviruses to introduce both luciferase and mCherry is a convenient strategy that allows expression of the markers in almost all mammalian cells and to easily determine transduction efficiency and access cellular responses.

LV-EF1α-NLS-GFP-Puro is a pre-made lentivirus which express eGFP fused with SV40 nuclear localization sequence (NLS) under the EF1α promoter with co-expression of puromycin selection in a IRES cassette. Ready to use format.

LV-EF1α-NLS-mCherry-Puro is a pre-made lentivirus which expresses mCherry fused with SV40 nuclear localization sequence (NLS) under the EF1α promoter with co-expression of puromycin selection in a IRES cassette. Ready to use format.

The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found in most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

MAGE (melanoma associated antigen) proteins are CT (cancer testis) antigens, and there are about 60 proteins in the MAGE family that can be subdivided into type I (present only on the X-chromosome, MAGE-A, B and C) and type II (MAGE D-L and necdin). Under normal conditions they are mostly found in the testis and placenta. They are found at high levels in several cancer types, such as melanoma, brain, and breast cancer, and are involved in the development of resistance to chemotherapy, cell motility and cell survival. Expression of MAGE proteins tend to correlate with a poor prognosis. They are intracellular proteins, with MAGE-A1 being mostly cytosolic, making them poor targets for strategies such as CAR-T cell therapy. MAGE proteins are degraded in the proteosome, and the peptides created can then be found on the cell membrane in combination with MHC (major histocompatibility complex) I. The presentation on the cell surface in this form makes them an attractive target for TCR (T cell